What is Virus Plaque Assay

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In this Video, Ms. Poonam Rajawat, Assistant Professor, Biyani Group of Colleges, describes about virus plaque assay. It is an assay to enumerate phage particle on solid agar media. A clear area formed on agar media known as Plaque. It is a double layered technique. Phage dilution was prepared in trypton broth and two drops of E. coli. were added when bacteriophage affect the bacteria, it causes lytic cycle and form plaque.

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