Month: August 2015

video on Chordata Classification

In this Video, Ms. Deepika Rathore, assistant professor, Biyani Girls College, Jaipur, explains about the initiation step of Eukaryotic DNA replication, in which the ORC recognize the ARS and when ABF1 binds at B3, melting starts at B2 with the help of helicase and T-Ag protein. The Okazaki fragments are formed by the pol α and the leading strand is formed by the Pol δ which is called pol switching.

The lecture includes DNA sequencing procedure by Sanger. The process involves the termination of DNA replication by the use of dideoxy nucleotides made of four different bases of DNA. It involves the DNA polymerisation by the PCR but the reaction mixture consist of primer, dNTPs, DNA template, buffer, DNA polymerase and ddNTPs consisting of pentose sugar lacking –OH group at 3′ position. The chain extension takes place till the DNA polymerase do not encounter ddNTPs. After PCR the products are analysed on AGE and finally sequenced from bottom to up.

In this video Ms. Aastha Pareek, Biyani Groups of colleges, Jaipur, explains about differences between Lanthanides and Actinides.

It is a part of lecture presented by Pragya Dhakar, Assistant Professor, Biyani Group of colleges. On behalf of Gurukpo this lecture discuss about the modes of nitrogen excretion in different life forms i.e. Ureotelic ,Ureicotelic, Ammonotelic and main emphasis has been given on the ureotelic mode wherein the amino nitrogen is removed in form of urea. Further urea cycle is discussed in detail.

In this Video, Ms. Ankita Maan, Biyani Groups of Colleges, Jaipur, is explaining about hypertension. Hypertension is a condition in which the arteries have persistently elevated blood pressure. The higher the pressure the harder the heart has to pump.

The lecture includes general about electrophoresis technique and its basic principals. The technique is specifically described for the separation of DNA molecules of different sizes. The technique is based on the principal that the charged molecules separate depending upon their charge to mass ratio. Further its procedure involves gel preparation in TE buffer, Solidification of gel and loading of sample and finally the visualization of DNA bands in UV illuminator.

In this Video, Dr. Leena Kansal, Assistant Professor, Department of Science, Biyani Group of Colleges explains about the plasmid .In this video, basic features, size and copy number and classification of plasmids has been discussed. There are main five types of plasmids that includes F plasmid, R plasmid, Col plasmid, Degradative plasmid and virulence plasmid.