Cell Biology

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Section-A The cell structural Organisation : Development of cell theory, Eukaryotic and Prokaryotic cells, the nucleus and cell cycle(Mitosis and meiosis). The ultra structure of the cytoplasm-the cytoskeleton, microtubules, microtubular, organelles, microfilaments, the Endomembrane system, nuclear envelop, endoplasmic reticulum & Golgi complex, membrane organelles, mitochondria, chloroplast, lysosome, peroxisomes. Section-B Transport Across Cell Membrane: Molecular organization of cell membrane, passive and active transport, Na-K pump, Ca2+ ATPase pumps, Lysosomal and Vacuolar membrane ATP dependent proton pumps, Co-trnasport into prokaryotic cells, endocytosis and exocytosis, entry of viruses and toxins into cells. Receptors and models of extra cellular signaling: Cytosolic, nuclear and membrane bund receptors, examples of receptors, autocrine, paracrine and endocrine model of action.Section-C Signal Transduction: Signal amplification, different model of signal amplification, cyclic AMP, role of inositol phosphatase messenger, Biosynthesis of inositol triphosphatases, cyclic GMP and Glycoproteins in signal transduction. Calcium models of signal amplification, phosphorylation of protein kinases. Section-D Call Culture: Techniques of propagation of prokaryotic and eukaryotic cells, cell line, generation of cell lines, maintenance of stock cells, characterization of cells, immunochemistry, morhpological analysis techniques in cell culiure, primary cultures contamination, differentiation, three dimensional cultures

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